The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques

نویسندگان

  • Huirong Lin
  • Guangcun Chen
  • Shenhai Zhu
  • Yingxu Chen
  • Dongliang Chen
  • Wei Xu
  • Xiaohan Yu
  • Jiyan Shi
چکیده

In order to investigate the microbe-mineral interaction in the micro scale, spatial distribution and speciation of Cu and S in Halothiobacillus HT1 biofilm formed on a CuS surface was examined using synchrotron-based X-ray techniques. Confocal laser scanning microscope (CLSM) results indicated that Halothiobacillus HT1 biofilm formation gave rise to distinct chemical and redox gradients, leading to diverse niches in the biofilm. Live cells were distributed at the air-biofilm and membrane-biofilm interface. CuS was oxidized by Halothiobacillus HT1 biofilm, and copper penetrated into the biofilm. Sulfide was oxidized to cysteine (77.3%), sulfite (3.8%) and sulfonate (18.9%). Cu-cysteine-like species were involved in the copper homeostasis. These results significantly improve our understanding of the interfacial properties of the biofilm-mineral interface.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Review of the Applications of Synchrotron Radiation in Archaeological Sciences

The scientific research regarding investigation, characterization and protection of the archeological specimens is manifested through a notable participation of multidisciplinary subjects and experts, scientists and archeometrists. One of the main principals which are considered by archaeometrists in the study of the precious specimens is the utilizing nondestructive methods. As an example, in ...

متن کامل

Interaction of primary mammary bovine epithelial cells with biofilm-forming staphylococci associated with subclinical bovine mastitis

Background: Staphylococci are recognized worldwide as one of the most important etiological agents of bovine mastitis due to their virulence factors such as their ability to penetrate inside mammary epithelial cells and their ability to form biofilm. Aims: The objectives of this study were to establish a model of primary mammary epithelial cells origin...

متن کامل

Detection of breast cancer using non-invasive X-ray diffraction technique of hair: A preliminary study

Background: An early diagnosis of breast cancer relates directly to an accurate treatment plan and strategy. Early detection of breast cancer before its development would be a significant reduction of morbidity and mortality rates. The aim of this preliminary study is to investigate the sensitivity of Wide Angle X-ray diffraction (WAXRD) method on women hair samples of healthy and breast cancer...

متن کامل

Effect of 900 MHz microwave radiation on alpha-int1 gene expression, proliferation and adherence of Candida Albicans

To date, registered users of mobile phone communication network exceeded from total numbers of the world population, while a little knowledge of the biological effects of, 900-1800 MHz microwave radiation, originating from the handsets or the base transceiver stations, have been released. The current study was designed for evaluation of 900-MHz radiation effects on Candida albicans proliferatio...

متن کامل

Application of Convolution of Daubechies Wavelet in Solving 3D Microscale DPL Problem

In this work, the triple convolution of Daubechies wavelet is used to solve the three dimensional (3D) microscale Dual Phase Lag (DPL) problem. Also, numerical solution of 3D time-dependent initial-boundary value problems of a microscopic heat equation is presented. To generate a 3D wavelet we used the triple convolution of a one dimensional wavelet. Using convolution we get a scaling function ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 14  شماره 

صفحات  -

تاریخ انتشار 2013